Streamlined multiphoton confocal for deeper, faster multiphoton imaging.

Nikon’s A1 MP+ multiphoton confocal microscope is a unique multiphoton imaging system featuring a high resolution galvanometer scanner. New 4 channel non-descanned detectors with higher sensitivity, reduced dark current and broad spectral range allow for real time unmixing of closely spaced probes for accurate and high-contrast spectral imaging. This is especially important in multiphoton microscopy because of the overlap of emission spectra of probes and autofluorescence, which is often unavoidable when using a single laser line.

Deep Specimen Imaging with High-sensitivity Non-Descanned Detectors (NDD)

The fluorescence emissions from deep within a specimen are highly scattered in multiphoton excitation, and therefore the conventional detector using a pinhole cannot provide bright fluorescent images. The episcopic NDD in the A1 MP+ is located close to the back aperture of the objective to detect the maximum amount of scattered emission signals from deep within living specimens.

In vivo image of deep areas of cerebral cortex of a mouse

The cerebral cortex of an H-line 5-week-old mouse was studied with the open skull method. The entire shape of dendrites of pyramidal cells in layer V expressing EYFP were visualized from the bottom layer into a superficial layer. In addition, the fluorescence signal of white matter in deeper areas was also studied.

3D volume rendering images

1300nm Imaging

Deep brain imaging in in vivo mouse with the GaAsP NDD compatible with the 1300 nm wavelength

Mouse brain in vivo dual color imaging with the GaAsP NDD compatible with the 1300 nm wavelength

Nikon High-NA Objectives are Ideal for Multiphoton Imaging

Auto Laser Alignment when Changing Multiphoton Excitation Wavelength

Increased flexibility and ease of use

for more information please visit :

https://www.nikoninstruments.com/Products/Multiphoton/A1MP-Multiphoton