Hybrid scanner for ultrafast photoactivation imaging

A1R+ has a hybrid scan head that incorporates both an ultrahigh-speed resonant scanner and a high-resolution galvano scanner. Simultaneous photoactivation and ultrafast imaging using these two scanners allow acquisition of rapid changes after photoactivation and enables observation of intermolecular interaction.

HeLa cells expressing Yellow Cameleon 3.60 were excited with 457 nm laser light. After stimulation with histamine, calcium ion concentration dynamics were observed. The (blue) emission of CFP and the (yellow) emission of YFP are shown as green and red channels respectively. The graph displays fluorescence intensity (vertical) versus time (horizontal). The green and red lines in the graph indicate the intensity change of CFP emission (green) and YFP emission (red) from the region of interest (ROI). Along with the increase of calcium ion concentration in the cell, the intermolecular FRET efficiency between CFP and YFP within Yellow Cameleon 3.60 increases, the CFP fluorescence intensity decreases, and the YFP fluorescence intensity increases.
Imaging laser wavelength: 457 nm, Image size: 512 x 512 pixels, 30 fps (with resonant scanner)
Photos courtesy of: Dr. Kenta Saito and Prof. Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University

What is a hybrid scan head?

This mechanism allows flexible switching or simultaneous use of two scanners (resonant and galvano) with the use of a hyper selector.

Ultrafast imaging with a resonant scanner

High-resolution imaging with a galvano scanner

Increased light detection efficiency realizes high image quality

Fast and accurate spectral imaging: A1-DUS Spectral Detector Unit

High-speed spectral imaging

Accurate and high-speed unmixing

Wide band spectral imaging

V-filtering function

Bright spectral imaging: A1-DUVB GaAsP Detector Unit

High-sensitivity spectral image acquisition

Variable acquisition wavelength range

Optional second channel detector

Accurate spectral unmixing

Simple operation of complex applications