Faster, deeper, sharper multiphoton confocal imaging

Nikon’s A1R MP+ multiphoton confocal microscope is a unique multiphoton imaging system featuring a fast, high resolution galvanometer scanner and an ultra-high speed resonant scanner that is capable of frame rates from 30 fps at 512 x 512 pixels to as fast as 420 fps in band scan mode. This is especially important inmultiphoton microscopy because of the overlap of emission spectra of probes and autofluorescence, which is often unavoidable when using a single laser line.

Resonant Scanner Enables Imaging Up To 420fps

Nikon's exclusive resonant scanner is capable of imaging a wide area at a much higher speed than a non-resonant scanner, making it possible for 420-fps imaging using point scanning technology. The NDD for multiphoton microscopy makes it possible to image fast and deep through the thickest specimens. Nikon's optical pixel clock system allows more stable and more evenly illuminated imaging— even at high speeds.

Visualization of intravital microcirculation
Blood cells in blood vessels within a living organism were excited by a femtosecond pulsed IR laser with the A1R MP+' ultrahigh-speed resonant scanner, and their movements were simultaneously captured in three successive fluorescence images at 30 fps (30 msec), with three separate color channels.
Three fluorescent probes are simultaneously excited and imaged–nucleus (blue), endothelium (green), and plasma (red). The long-wavelength ultrafast laser in combination with the ultrahigh-speed resonant scanner effectively reduces photodamage and makes time resolved multiphoton imaging of biomolecules possible.

Image resolution: 512 x 512 pixels, Image acquisition speed: 30 fps, Objective: water immersion objective 60x

Imaged with the cooperation of: Dr. Satoshi Nishimura, Department of Cardiovascular Medicine, the University of Tokyo, TSBMI, the University of Tokyo, PRESTO, Japan Science and Technology Agency

Revolutionary Hybrid Confocal Scan Head

in vivo High-Speed imaging

Deep Specimen Imaging with High-sensitivity Non-Descanned Detectors (NDD)

NEW Dual IR Beam Option Enables Simultaneous Two-Color Multiphoton Imaging

Nikon High-NA Objectives are Ideal for Multiphoton Imaging

Auto Laser Alignment when Changing Multiphoton Excitation Wavelength

Increased flexibility and ease of use

Two Types of Scanning Heads Enable High-speed, High-quality Imaging

for more information please visit :

https://www.nikoninstruments.com/Products/Multiphoton/A1RMP-Multiphoton