Scientists have overcome many live cell imaging challenges using advanced techniques such as TIRF, confocal, FRET, photoactivation and microinjection. At the center of it all is the Eclipse Ti-E, a powerful inverted microscope system that provides instant access to all of these techniques plus revolutionary Nikon CFI60 optics. The Eclipse Ti-E offers improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging.

Stable Time-lapse Imaging with Automatic Focus Correction System

The Ti-E is equipped with a unique Perfect Focus System (PFS) that automatically corrects focus drift in real time during a prolonged period of time-lapse imaging.

Improved Performance in Broader Wavelength Range

By employing the 870nm wavelength for the coverglass interface detection, near-infrared fluorescence dyes including Cy5.5 can be used.

Maintaining Focus at Greater Depths

Focus drift can now be corrected in a broader range of Z-axis planes than ever before. Maintaining focus at greater distances from the objective lens and at greater depths within the specimen is also possible.

Compatible with Plastic Dishes and Well Plates

In addition to glass bottom dishes, plastic dishes can be used with PFS.* The system is especially suited to high-throughput screening applications that involve multi-well plates.

High-speed Motorized Control and Acquisition

The operational speeds of motorized components such as the nosepiece, fluorescence filters and stage have been greatly enhanced, allowing high-speed screening image capture during multi-dimensional experiments.

High-quality Phase Contrast Images using High NA Lenses

The revolutionary external phase contrast unit enables the use of high N.A. non-phase contrast objective lenses for phase contrast imaging by the use of an external phase ring. 

Advanced Integration with Peripherals via NIS-Elements Imaging Software

Nikon's comprehensive imaging software NIS-Elements provides an integrated control of the microscope, cameras, components and peripherals.

Ti-LAPP Modular Illumination System

The new Ti-LAPP system provides a wide range of illumination modules that can be flexibly combined to create an imaging system tailored for your research. 

DMD Module

The DMD module enables photoactivation and photoconversion of a user-specified pattern and position(s), whereas the conventional FRAP unit only enables photoactivation of a single, manually-positioned spot.

H-TIRF Module

The H-TIRF module automatically adjusts the focus and incident angle of the laser for TIRF observation by monitoring the reflection beam. An even field of TIRF illumination is achieved with a gradation neutral density (ND) filter.

Flexible Module Combination

The Ti-LAPP system’s modularity and flexible configuration capability provide custom imaging solutions for individual research needs. Modules can also be easily exchanged or added to adapt to changing experimental needs, an important feature for labs with evolving research directions and multi-user, core facilities.

Two-tiered Configuration Capability

Use of an optional back port enables multiple wavelength FRET imaging with multiple cameras.

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https://www.nikoninstruments.com/Products/Inverted-Microscopes/Eclipse-Ti-E