Hepatitis B infection is recognized as a health problem all over the world. The causative agent of hepatitis B virus has a genome DNA of families that Hpadna viridae half and the ring is double-stranded. It has an 8 genotypes and 9 serologic type the name of the type for which it is known. Genotype D is most prevalent genotype in Iran (under the type of ayw3-ayw-ayw2). Hepatitis B virus in the blood stream to form spherical structures called Dane particle that contains a cover and a central cavity of the capsid proteins and nucleic.  In this sensitive period, with the presence of the antigen is detectable in the bloodstream. In patients with HBsAg in patients recover within 20-12 weeks after the onset of clinical signs or increase the amount of liver enzyme is removed from the bloodstream. Stability of the antigen in the bloodstream for more than six months, a condition called chronic carriers are created. HBsAg hepatitis B infection is an indicator that a major role in the diagnosis of acute and chronic identifying responsible.