HCV HCV) C) was recognized in 1989 from the family Flaviviridae viridae and hepatitis transmitted through blood transfusion. The virus contains a single-stranded RNA genome and a capsid, is a matrix and a sheath. Genetically six genotypes of hepatitis C virus core (major) and at least 80 subspecies is divided. According to studies, the most common genotypes 1a and 3a and 1b and 4, respectively, in Iran and in Iran infection rate is about 1% of the population. HIV transmission through blood and blood products and contaminated needles, contaminated instruments as well as the possibility of contamination by the tattoos or piercing or circumcision and hemodialysis devices are used there. Transmission from mother to child or through sex is very limited. Most people who suffer from acute HCV infection are asymptomatic or with mild symptoms such as fatigue, nausea and jaundice are. Before the first symptoms of the disease incubation period is between 15 and 150 days. The disease becomes chronic in about 80% cases of which 20-15% will lead to cirrhosis and liver cancer are 4-1% of this amount. Among the various methods which are used to diagnose this disease, the highly sensitive ELISA was used for initial review and screening. Positive examples of this method, Western blot confirmatory method should be tested and checked. The third generation ELISA kit has high sensitivity and specificity, and ability to measure specific antibodies against HCV is capable of.