Synonyms: IFN-γ, IFNG, IFG, IFI, Type II Interferon

Reactivity：Monkey

Assay range：31.25–2000 pg/mL

Sensitivity：18.75 pg/mL

Detection Method：Sandwich ELISA, Double Antibody

Size：48T, 96T

Storage：2–8 ℃ for 6 months

CV %（）：Intra-Assay: CV<10%；Inter-Assay: CV<12%

Note：For Research Use Only

IFN-γ(Interferon Gamma) Basic information

Interferon gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulated with phytohemagglutinin, and by others as a product of antigen-stimulated lymphocytes. It was also shown to be produced in human lymphocytes, or tuberculin-sensitized mouse peritoneal lymphocytes challenged with Mantoux test (PPD); the resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. Those reports also contained the basic observation underlying the now widely employed IFN-γ release assay used to test for tuberculosis. In humans, the IFN-γ protein is encoded by the IFNG gene. IFN-γ, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoan infections. IFN-γ is an important activator of macrophages and inducer of major histocompatibility complex class II molecule expression. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFN-γ is produced predominantly by natural killer cells (NK) and natural killer T cells (NKT) as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops as part of the adaptive immune response. IFN-γ is also produced by non-cytotoxic innate lymphoid cells (ILC), a family of immune cells first discovered in the early 2010s.

Monkey IFN-γ(Interferon Gamma) ELISA Kit test method

Feiyue’s Monkey IFN-γ(Interferon Gamma) Elisa kit is an ELISA reagent for detection of Neutrophil elastase in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of Monkey IFN-γ(Interferon Gamma).Monkey IFN-γ(Interferon Gamma) -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti- Monkey IFN-γ(Interferon Gamma) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Monkey IFN-γ(Interferon Gamma) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.